![]() ![]() BicD2 thus recognizes Nup358 through a ‘cargo recognition α-helix,’ a structural feature that may stabilize BicD2 in its activated state and promote processive dynein motility. Mutations in this region of Nup358 decreased the Nup358/BicD2 interaction, resulting in decreased dynein recruitment and impaired motility. ![]() Using nuclear magnetic resonance titration and chemical exchange saturation transfer, mutagenesis, and circular dichroism spectroscopy, a Nup358 α-helix encompassing residues 2162–2184 was identified, which transitioned from a random coil to an α-helical conformation upon BicD2 binding and formed the core of the Nup358-BicD2 interface. Here for the first time, we show that a minimal Nup358 domain activates dynein/dynactin/BicD2 for processive motility on microtubules. However, the molecular mechanisms of the Nup358/BicD2 interaction and the activation of transport remain poorly understood. ![]() Nup358 interacts with the dynein adaptor Bicaudal D2 (BicD2), which in turn recruits the dynein machinery to position the nucleus. I set out to put together a service I can spin up on demand, to distribute live streams from events, such as corporate meetings/presentations, public events etc.Nup358, a protein of the nuclear pore complex, facilitates a nuclear positioning pathway that is essential for many biological processes, including neuromuscular and brain development. PRNewswire/ - Media streaming becomes more powerful with the latest release of Helix Universal Media Server from RealNetworks. The web based configuration looks good on the surface, but after battling with configuration for an hour, I noticed that the workflow was not intuitive. After trial and error, I was able to get Adobe flash media live encoder to stream to the server, but because of the inane design, there is no way to preview the video or generate a working URI from the interface to receive video from the server. Instead, you are expected to create a URI in your head or programmatically, guessing what virtual directory names to insert to match the protocol you wish to use (rtmp, rtsp, HLS etc), no mention if order of operators is important. I wasted plenty of time, read all of the documentation and all relevant forum posts, and got NO RESULT, NOT A SINGLE FRAME OF VIDEO SERVED. Helix Universal Media Server (Linux/Unix). This applies to p4, p4d, p4p, and p4broker components of the server release. Because of the limited number of people using this software and the specialised skill set of people in the industry, there is very little knowledge about the use of this software published to the web, which makes vendor documentation vital. The following 32-bit platforms are now in the Obsolescent phase: Linux kernel 2.6+. It seems that making the software easy to use is not important to the vendor. Helix Universal Server is a program that streams live and on-demand media. Amazon held up their end, the AMI required minimal time to deploy. Review Comments Questions & Answers Update program info. I highly recommend that people try this software out, the barrier for entry is low. ![]() The Helix Universal Server, Helix Producer and Helix Enterprise Player are no longer available for license from any supplier. Much cheaper to test out than the competition. Adlib, Express and Express Server are either registered trademarks or trademarks. Support Live, agent-assisted support is no longer available. Just prepare yourself for disappointment.Ĭloud Print web printing service is a trademark of Google Inc. ![]()
0 Comments
Leave a Reply. |